This is a transverse image obtaining a cross section through the lower lip and jaw. The image demonstrates an intact lower jaw line. The width of the mandible should be similar to the width of the maxilla and may be reduced in micrognathia. Again tooth sockets are apparent.

When measuring the nuchal fold angling the probe to place the falx at ~15° to horizontal may provide a sharper image of skin line and bone. This may minimise the chance of beam width artefact causing a thickened nuchal fold measurement.

This is a transverse section of the abdomen inferior to the abdominal circumference. The best views are obtained with the cord insertion near the three or nine o'clock position. The skin line should be clearly visualised on both sides of the insertion. The base of the cord should insert cleanly into abdominal wall with no evidence of associated mass to suggest gastroschisis or omphalocele.

Doppler settings need to be sufficient to demonstrate the physiological atrial septal defect of the foramen ovale and complete filling of the ventricles. 31

MSKultrasound

The crux of the heart demonstrates an offset cross arrangement of the AV valves. The right valve (tricuspid) is closer to apex than the left (mitral).

The axis of each kidney is roughly parallel to the aorta; if they angle towards each other at the caudal ends then this may indicate a horseshoe kidney. Each kidney should be similar sized (20–22 mm length in the second trimester) with no renal cysts visible.

Doppler can be used to demonstrate no turbulence across aortic valve and to ensure that there is the correct direction of flow across this valve.

Cordes Technique. The operator places his left hand in front of the screen with the palm on the spine and the fingers pointing at the sternum – the thumb of his left hand now points to the left side of the fetus.

The superior vena cava and IVC can both be seen entering the right atrium; this can be confirmed with color Doppler assessment.

There is occasionally some fluid in the pericardial sac where a thin rim is likely to be physiological (up to 2 mm). 30 Color Doppler is best performed with the septum between 45° and horizontal. This allows for improved Doppler detection of flow.

The nuchal fold is a measurement taken from outer skin line to outer bone in the midline (following an imaginary continuation of the falx). Less than 6 mm is considered normal up to 22 weeks.

It is advisable to scan through the placenta to attempt to find where the cord inserts into the placental mass. 47 This is easy with an anterior placenta but can be difficult to locate if the fetus is lying on a posterior cord insertion. If the cord inserts to the margin of the placenta then it should be reported as a marginal insertion and it is helpful to specify the side of insertion (e.g. upper left, lower right). If the cord inserts into the uterine wall and vessels then course around the wall to insert into the side of the placenta then this is described as a velamentous insertion. Either marginal or velamentous insertions can increase the risk of succenturiate lobes and vasa previa. These abnormalities should be scanned for carefully. 48

The axial images presented here are based on the five short axis views 29 (Figures 17–24). These views can also be assessed with color and/or power Doppler. Sagittal views of the chest can be also used to show the fetal arterial vessels (Figures 25–28). The heart rate should be noted throughout the scanning time to look for arrhythmias and can be recorded (Figure 29). Extra views of the systemic venous return to the right atrium can be readily obtained (Figure 30).

Vascularultrasound

Introduction: The second trimester ultrasound remains an important screening tool for detecting fetal abnormalities. This pictorial guide for the second trimester ultrasound is designed to assist practitioners to produce a high quality diagnostic survey of the fetus by demonstrating and describing recommended images.

This image is obtained by taking a mid‐sagittal view through the cervix. Ensure that the cervical canal can be seen as a hypoechoic line. The closest edge of the placenta should be located; sometimes this is not in the mid line but situated more laterally. The distance from the lowest edge of the placenta to the internal os should then be measured. A low lying placenta is suggested if the placenta lies less than two cm from internal os. 49 Accurate measurements cannot be obtained with a contraction of the myometrium occurring and time must be given for this to relax. Many centres will perform a transvaginal scan to assess the placental site more accurately if there is any doubt about placental position. 50 Color or power Doppler over the internal os is a quick technique to exclude a vasa previa. Occasionally the cord is draped over the cervix and this can lead to erroneous diagnosis of vasa previa, it is worthwhile checking again after asking the mother to roll from side to side to see if the cord moves to a different position.

There is a single vessel (descending aorta) behind heart just to the left of the midline (as defined by the ossification centre of the spinal body).

This image is a transverse section of the upper part of the fetal abdomen. This view is directly inferior to the 4–chamber view. In difficult cases it can be obtained by placing the fetal spine horizontally across the screen with the stomach visible in the centre of the screen and then rotating the probe 90°.

The abdominal circumference is a standard biometry measurement 6 , 7 (Figure 31). The umbilical cord insertion should be imaged to look for abdominal wall defects (Figure 32). The renal tract is reviewed by imaging urine in the bladder with surrounding umbilical arteries (Figure 33) and assessing the kidneys. The fetal kidneys should be imaged in 2 planes, both coronal and axial (Figures 34 and 35). Although not essential, visualising the renal arteries can help with kidney identification especially if unilateral renal anomalies are suggested (Figure 36).

The normal baseline fetal heart rate in the second trimester is 120–160 beats per minute. A regular rhythm should be demonstrated.

There are a number of measurements to take during the examination. Some measurements of fetal size should be included in the formal report of each examination. The minimum measurements to report are: biparietal diameter (BPD), head circumference (HC), abdominal circumference (AC), and femur length (FL). 5 – 7 Other biometry which could be reported include: humerus length (HL), nasal bone length (NB), nuchal fold (NF), cerebellar diameter (TCD), cisterna magna and cervical length. A combination of BPD and HC measurements can be used to calculate an estimated date of delivery (EDD). 8

Ultrasoundsmallparts

It is important to establish situs. Both the heart and stomach should be seen to be on the left side of the fetus. Establishing situs can be confusing, especially for learning practitioners due to variable fetal positions.

This is a coronal image of the back of the face, through the orbits. The orbits should be equal size with the gap between each orbit approximately the same as the width of each orbit (dividing the face into thirds). The lenses can be seen as central circles that should not have internal echogenicity. Lens opacity may indicate congenital cataracts.

The cerebellum is a dumbbell shaped structure, with symmetrical lobes. The central vermis is slightly more echogenic than the lateral lobes.

This is a mid‐sagittal image through the fetal head. A mid sagittal plane can often visualise most of the length of the corpus callosum. Visualisation of the corpus callosum can be facilitated by trying to angle the probe so as to scan through the area of the anterior fontanelle. The corpus callosum connects the left and right lobes of the brain. It sits superior to the cavum septum pellucidum and extends backwards as a hypoechoic line. The presence of the corpus callosum can be confirmed with color Doppler of the pericallosal artery. 18

The second trimester ultrasound is commonly performed between 18 and 22 weeks gestation. Historically the second trimester ultrasound was often the only routine scan offered in a pregnancy and so was expected to provide information about gestational age (correcting menstrual dates if necessary), fetal number and type of multiple pregnancy, placental position and pathology, as well as detecting fetal abnormalities. 1 Many patients now have several ultrasounds in their pregnancy with the first trimester nuchal translucency assessment becoming particularly common. 2 The second trimester ultrasound is now less often required for dating or detection of multiple pregnancies but remains very important to detect placental pathology and, despite advances in first trimester anomaly detection, remains an important ultrasound for the detection of fetal abnormalities. In order to maximise detection rates there is evidence that the ultrasound should be performed by operators with specific training in the detection of fetal abnormalities. 3

This sagittal image demonstrates intact diaphragms on each side especially posteriorly near the spine, a common site for diaphragmatic defects. The stomach is visible beneath and heart above the diaphragm. It is also beneficial to identify homogeneous appearing lung fields to attempt to exclude echogenic or cystic lung lesions.

Official websites use .gov A .gov website belongs to an official government organization in the United States.

Renal arteries. Coronal section with the aorta horizontal facilitates demonstrates of flow from the aorta to each renal artery.

This is a coronal section through the back just anterior to the fetal spine. A kidney should be seen on each side of the aorta. Each kidney is ‘C’ shaped around a single renal pelvis.

Measure the femur horizontally across the ossified diaphysis down the middle of the shaft of the bone avoiding any triangular echogenic extensions (seldom seen before the third trimester). A measurement less than the 2.5th centile may increase the risk of aneuploidy and raise concerns about skeletal dysplasia. 38

This is a mid‐sagittal view of the fetal chest, best obtained with the fetus lying supine. Shadowing from the spine can make this a difficult view to obtain when the fetus is prone. The ductal arch is directed straight back towards the spine with no head and neck vessels arising from it. The ductus arteriosus is continuous with the descending aorta. In contrast to the curved appearance of the aortic arch there is a straight ‘hockey stick’ appearance to the ductal arch. The pulmonary valve can often be visualised opening and closing. No head or neck vessels can be seen arising from this arch.

A ‘V’ shaped ‘arrowhead’ of aorta and ductus is made at their meeting point; the aorta is now directed towards the left shoulder. The width of the ductus is slightly larger than that of the aorta. In approximately 1% of fetuses an aberrant right subclavian artery can be seen passing behind the trachea towards the right shoulder, this has been associated with an increased risk of trisomy 21. 34

If a measurement of cervical length is requested (usually because of a risk factor for cervical incompetence), or the cervix appears short on abdominal views, the cervix should be assessed transvaginally with an empty bladder. 51 The operator should aim to obtain a mid‐sagittal view of the cervix demonstrating the hypoechoic cervical canal. Withdrawing the vaginal probe a short way is useful to ensure the probe is not pressing on the cervix as pressure on the cervix may artificially elongate the cervical measurement and may prevent opening of the canal with fundal pressure. The cervix should be measured with and without fundal pressure. 52 The shortest length measured with fundal pressure is the most important measurement. 51

The standard axial fetal brain planes include the biparietal diameter, the transventricular plane and the cerebellar plane (Figures 1–3). Many of the fetal measurements are taken from these planes including the BPD and HC. Measurements of the Cerebellum, Cisterna Magna and Nuchal fold can be useful. Further images of the head which may add value include the mid sagittal plane to view mid line brain structures, coronal cerebellum and coronal face (Figures 4–6).

The facial structures can be examined both coronally and axially. The orbits, nose and mouth need to be separately visualised (Figures 7–11). Areas which warrant particular attention are the size and spacing of the eyes as well as the upper lip (which is best imaged in two planes). There are a number of ultrasound techniques which have been suggested to improve detection of facial abnormalities including measurement of facial angles and 3D ultrasound techniques, 20 , 21 these techniques are usually employed when there is a suspicion of an abnormality rather than in the routine setting and are beyond the scope of this paper. Shortening (hypoplasia) of the nasal bone has been associated with Down syndrome 22 (Figure 12).

Ultrasoundmachine

The cord inserts centrally into an anterior placenta. Identification of the insertion point is facilitated by the use of color or power Doppler.

Doppler Assessment should aim to demonstrate complete filling of the aorta with color, and forward flow away from the heart with no obvious turbulence. Filling of the three head and neck vessels is ideal but not always obtainable.

Four‐chamber with blood flow – intraventricular septum. Color flow is seen into both ventricles, all of the way to the apex. Flow is demonstrated across the foramen ovale.

The lateral ventricular measurement can be taken from inner wall to inner wall at the level of the glomus of the choroid plexus. The lateral ventricle should be measured at right angles to the falx. 12 Over the gestational range 15 to 40 weeks 10 mm or larger is considered abnormal. 14

Color or power Doppler over the internal os may help exclude vasa previa (Figure 52). If there is any suspicion of shortening of the cervix or low lying placenta then a transvaginal scan often provides more accurate measurements43 (Figure 53). Many centres will perform a transvaginal scan to assess the placental site and cervix more accurately if there is any doubt about placental position.

This is a coronal section through the thorax and abdomen. The heart and stomach are observed to be both left sided with the diaphragm as an intact line between.

Result: The authors hope this article may act as a useful guide to all practitioners performing second trimester ultrasounds.

What isultrasound

Ultrasound partsand functions

Both hemidiaphragms can be visualised sagittally (Figures 14 and 15). The lung fields should be carefully inspected for cystic or echogenic areas.

Because of the risk of artefacts it is often useful to visualise the major structures in at least two planes. The measurement techniques recommended here are widely accepted techniques, however some charts may use different techniques and each operator should measure in accordance with the charts agreed within their own work place and population.

Each of the twelve long bones should be separately visualised (Figures 42–47). Although both femurs could be measured it is probably sufficient to only measure one provided both have been seen to be of similar lengths. When imaging the long bones the operator should ensure they are not angulated or bowed, that they are echogenic and are of an appropriate length. If there is doubt about long bone length then they can each be individually measured and checked against standardised charts. 36 Hands and feet should be separately imaged taking particular care to ensure that both left and right sides are separately seen (Figures 45 and 48).

Articles from Australasian Journal of Ultrasound in Medicine are provided here courtesy of Australasian Society for Ultrasound in Medicine

This is a transverse image used to obtain a cross section through the upper lip and hard palate. It demonstrates an intact skin line of the upper lip (no cleft). Behind this is an echogenic intact hard palate; this confirms the correct plane, and is useful to distinguish palate involvement if a cleft lip is present. The hard palate has internal areas of reduced echogenicity representing tooth sockets. This image does not exclude cleft of the soft palate or secondary hard palate.

Slight gaps in the echogenic skull bone outline are evident and represent the skull sutures. There should be a normal oval skull shape with no depression of the petrous temporal bones and no angulation near the sutures. The normal bone density of the skull should be more echogenic than the falx.

There should be no frontal bossing – no forward sloping of the forehead. The tip of the nose, upper lip, lower lip, and chin should line up along the same imaginary line. If the chin is significantly behind this imaginary line then micrognathia is suspected. 26

The male genitalia are best assessed by taking two images. Firstly a mid‐sagittal image of the lower abdomen below the cord insertion demonstrates penis and scrotum caudally to the cord insertion. Secondly a transverse image, just below the level of the bladder, best taken with the knees separated. Penis and scrotum are seen between the thighs in this image. Testes are undescended at this gestation, and usually descend later in the third trimester.

The calcified bone is actually the femoral diaphysis, the epiphyseal ends do not calcify until later in pregnancy. The image should be taken with the femur horizontal (perpendicular to the ultrasound beam). 37 This makes it easier to see the full extent of the bone and avoid foreshortening. When the full length of the femur is on the screen there is often a posterior acoustic shadow noted.

Placing a Doppler box over the internal os is useful to exclude vasa previa (Figure 52). One must be sure that any vessel seen is not just a mobile loop of umbilical cord. If a vasa previa is found an attempt at locating a succenturiate lobe should also be made.

The stomach should be visualised in the left side of the abdomen. A ‘J’ shaped hypoechoic structure is seen in the midline; it should be 1/3 of the way across the abdomen and represents the internal portion of the umbilical vein branching to the right portal vein.

Two long bones should be seen in each leg. The tibia and fibula are of equal length. A sagittal side on image of each leg will demonstrate the ankle to be correctly orientated. In order to exclude talipes the heel should be ‘square’ with the sole of the foot orientated at right angles to the leg bones. The sole of the foot and the length of tibia and fibula should not be able to be seen in the same image.

This pictorial guide is provided as, despite a large volume of literature on the subject, it is difficult to find a single publication that describes the landmarks and range of images which are most useful to look for when performing the second trimester ultrasound. These images were all obtained on GE Healthcare Voluson E8 or 730 machines (GE Healthcare, Sydney, Australia). The images provided are representative. Some examinations may yield significantly better images while other examinations, especially in large patients, may yield much less clear images. The authors are not suggesting that an examination is only complete if all of these images are presented. Rather, we are suggesting that each labelled landmark is worth examining carefully during a second trimester ultrasound. Although the important features are described it is beyond the scope of this article to discuss the associated pathologies of each feature.

Normally both bones terminate at the same level at the wrist but the ulna is longer than the radius by 2–3 mm at the elbow. Both ulnas are lower in the images with wrists on the right of each image. It is important to identify the full length of the radius as radial ray defects can occur. 39

Still in an axial section move further in a cephalic direction from the four‐chamber view. The pulmonary trunk arises from the right ventricle close to the sternum, it is directed backwards towards spine. 32 The pulmonary trunk divides into the ductus arteriosus which continues directly towards the spine in the midline; and the right pulmonary artery which curves behind ascending aorta and SVC. The left pulmonary artery is not usually visible in this plane; it is directed in a more inferior direction. Bifurcation of the pulmonary trunk into ductus arteriosus and right pulmonary artery is important to establish that the vessel arising from the right ventricle is going to the lungs.

The heart rate can be observed throughout the fetal heart evaluation. Formal measurement of the heart rate is only a snapshot and there is considerable heart rate variation, especially with fetal movements. Heart rate can be measured with M‐mode or pulse wave Doppler. Pulse wave has an added benefit of allowing the mother to heart the heartbeat but involves higher energy levels. Extra information can be gained by placing the M mode through both a ventricle and atrium to demonstrate the heart rate in both structures.

This is a transverse section of the abdomen at the level of the bladder. Fluid should be seen within the bladder at some stage during the examination. A large bladder may be ‘keyhole’ shaped and may indicate urethral obstruction (partial or complete).

The authors have provided a selection of images which we feel are suitable for a comprehensive screening examination. Other images will be required if an abnormality is suspected or detected. Prenatal ultrasound is a rapidly developing field and other structures may become important in future years and these may require different or extra images.

This image is obtained by moving the scanner forward from the coronal orbital view to the very front of the face. This image demonstrates two nostrils and a separate intact upper lip. Being able to visualise the tip of all four structures (the nose, upper lip, lower lip, and chin) in the same coronal image makes micrognathia an unlikely finding.

The whole spine should be assessed in transverse section, this is best done by running up and down the spine in real time but representative images of each part of the spine can be taken. The three ossification centres form an approximately equilateral triangle throughout the length of the spine. Spreading of the laminae to form a more obtuse angled triangle may indicate open spina bifida. The operator should look for an intact skin line over the spine throughout, taking particular attention to ensure that there are no cysts or masses visible on the back.

This is a coronal image through the back of the fetal head. This image may be useful to demonstrate normal depth of the cerebellar vermis. The cerebellar lobes should be equal size. The vermis of the cerebellum should be more than 1/2 the height of the lobes. 19

Left and right sides of the heart should be approximately equal size. Both left and right ventricles should extend to the apex of the heart and should be approximately equal in width at the level of the atrio‐ventricular (AV) valves.

The humerus is best measured with it lying horizontal across the screen (perpendicular to the sound beam). 37 This makes it easier to see the full extent of the bone and avoid foreshortening. Less than the 2.5th centile may increase the risk of aneuploidy and raise concerns about skeletal dysplasia. 38

Secure .gov websites use HTTPS A lock ( Lock Locked padlock icon ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

This is the highest transverse plane for evaluating the fetal heart. Move the transducer further in a cephalic direction from the right ventricular outflow tract view, maintaining an axial section. The three vessels are, from left to right: the top of the pulmonary arch, the aortic arch and the SVC. 33 The trachea lies to right of the aorta and behind the SVC and has echogenic walls compared to the other vessels.

It is beneficial to look carefully at the CSP to ensure it is distinguished from the third ventricle and to identify the corpus callosum. Just anterior to the CSP there is often a fine hypoechoic ‘U’ shaped structure representing the anterior leaflets of the corpus callosum. The CSP is an important landmark for development of the corpus callosum, if it is not visualised then there is a risk of a range of brain abnormalities. 15

This plane is inferior to the BPD plane with the probe tilted backward into the posterior fossa. The plane is correct when one can visualise the thalami and cavum septum pellucidum in the same plane as the cerebellum. 12

Ultrasoundprobe

This is a sagittal midline section; best views are obtained if the fetus is prone. There should be an intact skin line i.e. a continuous skin line overlying the back, especially over the sacral region. There should be no spinal angulation or deformity. At the inferior end there should be sacrococcygeal tapering of the spine.

Demonstrates flow away from the heart with no obvious turbulence or reverse flow and no tributaries directed towards the neck.

It is useful to have some record of the examination for future reference. Video clips or DVD recording of the scan has the advantage of providing moving images, which is particularly helpful when assessing the fetal heart. A series of still images is however easier to store and refer to in the future. Images should clearly display identifying information such as the patient's full name; birth date; medical record or identification number; date of the ultrasound examination; and site where the examination was performed (hospital or private practice), 4 ensuring compliance with local legal requirements.

This is an optional image not frequently included in the routine examination but it is easy to obtain and is useful when evaluating potential abnormalities. The image is obtained by a para‐sagittal section just to the right of the mid line and just to the right of the ductal arch view.

These arteries need to be traced around the bladder towards the cord insertion to differentiate them from the iliac vessels that are seen more laterally in the pelvis directed towards the thighs. It is important to ensure that the Doppler settings are suitable for low flow to maximise the ability to detect both arteries.

This is a transverse section of the fetal face though the orbits. The face needs to be looking up for best views. The orbits should be approximately equal size and should be evenly spaced. The width of the nasal bridge between each orbit is approximately the same as the size of each orbit – dividing the face into thirds.

This is a coronal image through the back designed to more carefully assess the base of the spine. Sacrococcygeal tapering of the spine is normal; the sacrum extends just below the iliac wing. Separation of the lateral ossification centres or lack of visualisation of the central ossification centres may indicate a spinal defect. Occasionally the central ossification centres are not seen due to shadowing artefact from the adjacent iliac wing, angling away from a true coronal image to an oblique coronal image should help avoid this shadow artefact.

This guide is presented roughly in cephalic to caudal order, but where possible grouped by organ system. Operators would benefit from a systematic approach to ensure that all structures are seen even in difficult circumstances, as it can be possible to miss a structure, especially when there are active fetal movements.

The BPD measurement is obtained from outer skull bone to inner skull bone (leading edge to leading edge), perpendicular to the falx at the maximum diameter. 12 The HC is measured as an ellipse around the outside of the skull bones. Both of these measurements can be used to confirm gestational age. A head circumference measuring less than 3 standard deviations from the mean may indicate microcephaly. 13

This image is a cross‐section of the head just above the BPD‐ plane, at the level of the atrium of the lateral ventricles but still with the CSP in view. The image usually demonstrates a substantial length of choroid plexus superior to the level of the thalami.

The placenta is quite uniform in appearance at this gestation. Small hypoechoic vascular lakes can appear and do not appear significant. 44 These usually will not show flow with power Doppler. Large lakes, greater than 2 cm by 2 cm, may increase the risk of small for gestational age in the third trimester. 45 The placenta should be less than 4 cm thick. 46

The placenta should be visualised throughout to look for abnormalities, placenta previa, or hemorrhages (Figure 50). The operator should check the entire internal surface of the myometrium to ensure that there is no succenturiate lobe. The position of the placenta needs to be established to see if it is close to the internal os (Figure 51). The maternal cervical canal can also be measured, preferably with an empty or near empty maternal bladder (Figure 51).

Smallparts ultrasoundPDF

Many machine pre‐sets are a compromise of resolution and persistence and it is important to have a high resolution pre‐set available. A separate pre‐set with higher frame rates and higher contrast is useful for cardiac images. When taking images the structure of interest should occupy about 75% of the screen to maximise resolution. Write zoom (pre‐acquisition) is the zoom mode of choice as it has higher resolution than read zoom (post acquisition).

Three vessels and trachea view (3VT), Doppler assessment. The correct direction of flow (away from the heart) is demonstrated in both the aorta and ductus Arteriosus.

This is a transverse section of the abdomen. Placing the spine uppermost obtains the best image. The kidneys can be seen on either side of the spine.

Methods: Each image is discussed in detail and has an associated drawn line diagram to aid in the identification of the important features of that image. There is a description of the salient landmarks and relevant measurements.

The Abdominal Circumference is measured around the outside of the skin line. It is important that this section is correctly obtained to minimise measurement error. A number of features can be used to assess if the section is correct: The section should be circular not oval; the kidneys should not be visible in the section; the cord insertion should not be visible; and the ‘J’ should not extend all of the way to the skin line anteriorly (umbilical vein should not be visible to out to the skin line). If these features are incorrect then the section may be oblique and the AC may be overestimated.

RVOT Doppler assessment. No turbulence is seen across the pulmonary valve, the direction of flow down the ductus arteriosus is correct.

The heart is a difficult organ to assess due to the size, rapid movements and large number of small parts. It is unfortunately also a common site for abnormalities. This is an area of the examination which is best done in real time or saved as a video clip. As well as the internal cardiac structures the examiner must be sure that the heart is correctly positioned on the left side of the chest, with the interventricular septum at about a 45° angle and is of normal size.

The trans‐cerebellar diameter is the widest measurement across the cerebellum, perpendicular to the falx. Cerebellar size in millimetres correlates with gestational age up to 20 weeks and is larger than gestational age after this time. A cerebellum measuring 2 mm less than gestational age is a concerning finding. 16

This is a mid‐sagittal image best taken with the angle of the face at about 45°. 22 The skin line over the nose should be close to horizontal. This is a slightly more laid back angle than the corpus callosum view. A slight gap between the nasal bone and the frontal bone should be visualised to help identify the extent of the nasal bone.

This is a coronal image through the back. Often more than one image is required to see the whole spine. The sacrum extends just beyond the iliac wing. Tapering of the spine in the sacrococcygeal region is normal. The examiner should carefully assess for angulation or deformity of the spine and ensure that no hemivertebrae are seen. A slight expansion of the width of the lumbar spine is common.

This is an oblique sagittal view. The aortic arch arises from the middle of the heart and has a curved ‘walking stick’ shape to its arch. Three head and neck vessels arise from the aorta. The arch can be seen to be continuous with the descending aorta with no arch interruption or narrowing to suggest coarctation.

Color or power Doppler is used to identify the two umbilical arteries that surround the bladder and then are directed towards the cord insertion.

As with the male genitalia the female external genitalia are best assessed by looking from two directions. The first image is a mid‐sagittal image of the lower abdomen demonstrating the flat mons pubis caudal to the cord insertion. Secondly a transverse image, just below the level of the bladder, best taken with the knees separated. No penis is seen between the thighs, rather there are three lines representing the labia visible.

Each hand needs to be separately imaged. Hands need to be seen to open fully at least once during the scanning time, and should be seen to open at least once. If the hands remain clenched throughout the scan then there is a risk of trisomy 18. 40 The thumb should not be persistently adducted. 41

This is an axial view through the lower part of the fetal chest. The best B‐mode images are obtained with the apex orientated superiorly. Obtaining a full length of a rib on each side is a good technique to avoid an oblique section.

This image is a transverse section through the upper abdomen just below the four‐chamber view of the heart. It is the same section or just above the level of the abdominal circumference. The stomach is a left sided cystic structure that should not cross the mid line to the right side.

The cisterna magna can be measured from the posterior margin of the cerebellar vermis to the inside of occipital bone in the midline (following an imaginary continuation of the falx). A measurement of 2–10 mm is normal in the second and third trimesters. 17

From the four chamber view rotate the probe slightly towards the right shoulder and move the probe slightly in a cephalic direction. 32 This plane will demonstrate the aorta arising from the left ventricle; its medial wall must be seen to be continuous with the interventricular septum to exclude aortic override. Often the aortic valve can be seen opening and closing in the centre of the vessel. The ascending aorta is directed towards the right shoulder and turns back to the left at a higher level to form the aortic arch.

Each hypoechoic renal pelvis can be measured in the anterior posterior direction. The normal renal pelvis measures ≤ 4 mm in the second Trimester. 35

This is a coronal section through the back just anterior to the fetal spine, the same section as Coronal Kidneys. Low flow settings are needed to detect both arteries. The arteries should extend all of the way into the renal pelvis.

The full length of the echogenic calcified nasal bone can be measured. The 2.5th centile for the nasal bone measurement has been reported as 4.4 mm at 18 weeks and 5 mm at 20 weeks. 23 Other authors have used the 0.75th multiples of the median (MoM) being 3.6 mm at 18 weeks and 4 mm at 20 weeks. 24 A hypoplastic nasal bone has been associated with an increased risk of Down Syndrome. 25

This image is used to ensure that there are a correct number of toes on each foot. The leg should not be visible in same plane as the sole of the foot. The second toe can be longer than the great toe. There should be no cleft in the foot. The toes are best counted when they are pointing up towards the transducer.

The aorta sits just in front of the spine, slightly to the left of the spine. The inferior vena cava (IVC) is anterior to the aorta and right sided.

A review of the uterine wall looking for fibroids or bicornuate uterus and a review of the adnexae for ovarian cysts is suggested (these images are not presented in this publication).

Occasionally the gall bladder is visible as a tear shaped hypoechoic structure situated to the right anterior of the umbilical vein. The Adrenal glands can sometimes also be seen in this section. There should be no cystic dilatation of the bowel or abdominal cysts visible.

It is beneficial to image the fetal spine throughout its length and the spine is best imaged in three planes: coronal, sagittal and axial (Figures 37–40).

Not all departments will require each of the images presented in this manuscript to be obtained at each ultrasound. The authors are not suggesting that a second trimester examination is only complete if all these images are presented. Rather the authors hope that presenting these labelled images will help with education and understanding of the important structures, which can be visualised at a second trimester anatomy survey. It may not be possible to see every structure in some patients due to fetal position or maternal size. This manuscript may find value as a pictorial reference in imaging departments.

It may be advantageous to measure the near field ventricle as well as the far field lateral ventricle. Angling the probe to place the falx at ~15° to horizontal may facilitate visualisation of the near field ventricle.

DISCLAIMER PLEASE NOTE: Images, Descriptions, and Titles may not accurately represent the product. You are encouraged to confirm that the manufacturer item code above matches the products you are seeking. All patients or home users should have a valid prescription for any medical supplies or devices that require one. Some of our products have special shipping requirements due to weight and refrigeration; please visit our Terms of Service for more Details. All products on this website are subject to current manufacturer pricing changes and availability. Wilburn Medical Reserves the Right at any time to Cancel any Orders or to Not Fulfill Items on an Order that does not make good business sense.  Some dropship items are subject to Additional Taxes not calculated by this website. If you are uncertain or have questions, Please E-mail or Call us at 1-877-945-2876 for additional information.

Fetal movements should be observed and commented upon. It is essential to see flexion or extension of a limb at least once during the scanning process to reduce the chance of missing a case of arthrogryposis. 10 Similarly the opening of the hands to exclude clenched fingers is important to rule out several syndromes. 11

Rarely genital abnormalities are seen prenatally and therefore it is useful for the sonographer to examine this area. Some departments will allow parents to know the gender of the fetus. If the external genitalia are to be visualised for sex determination then imaging in both mid‐sagittal and axial planes is recommended to minimise error 42 (Figures 48–49).

Amniotic fluid assessment is usually subjective 9 but maximum vertical pocket or amniotic fluid Index (AFI) can be used if there is concern about excess or insufficient amniotic fluid.

This is a cross section of the fetal head obtained at the level of the thalami. The cerebellum, orbits and ears should not be visualised in this scanning plane. The falx should be positioned horizontal and equidistant from both parietal bones to avoid acynclitism (head tilted to one side). The operator should look for a symmetrical appearance to both hemispheres. The continuous midline echo representing the falx is broken in the anterior third by the cavum septum pellucidum (CSP). Behind this in the middle of the falx a thin slit representing the third ventricle is often visible.

One method to confirm visceral situs (sometimes referred to as the Cordes technique) has been demonstrated to be useful in normal and abnormal situations. 27 The technique is to orientate the fetal head to the right side of the ultrasound screen with the fetus lying horizontally across the screen. From this starting position, rotate the transducer 90° clockwise to obtain a transverse image of the fetus through the fetal heart. If the left hand of the operator is placed in front of the ultrasound screen positioned as an ‘L’ shape with the tips of fingers pointing towards the fetal sternum and the palm placed on the fetal spine, the thumb will now be pointing towards the left side of the fetus.28