İsmail Yener Dicle University Faculty of Pharmacy Department of Analytical Chemistry 21280- Diyarbakır, Turkey 0000-0002-0988-9462 Türkiye

In this study, the whole parts of eight edible and medicinal species (Alcea rosea, Carduus pycnocephalus subsp. albidus, Euphorbia gaillardotii, Euphorbia macroclada, Hypericum triquetrifolium, Kickxia lanigera, Malvella sherardiana and Mentha longifolia subsp. noeana were determined for their trace element (Al, As, B, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Se) contents using ICP-OES. Before the whole plant parts were analyzed by ICP-OES, the porcelain crucibles were ashed and dissolved by adding concentrated nitric acid and hydrogen peroxide. The accuracy of the method was assessed by NCS ZC73014 Tea Leaves. The trace element contents obtained from each samples were compared. Calibration curves had good linearity in the concentration ranges 0.05–1.5 mg L-1 for whole elements worked. Since correlation coefficient (R) values were found above 0.9997, the linear range was considered acceptable. When the studied species are compared in terms of their metal contents; Cd (0.41±0.02 mg kg-1) and Cu (13.9±0.9 mg kg-1) metals in Carduus pycnocephalus subsp. albidus, B (22.4±1.6 mg kg-1) metal in Euphorbia macroclada, Se (0.26±0.01 mg kg-1) metal in Hypericum triquetrifolium Al (1424±109 mg kg-1), Cr (4.8±0.2 mg kg-1), Fe (980±67 mg kg-1) Mn (44.6±2.4 mg kg-1) Ni (7.5±0.3 mg kg-1) and Pb (1.15±0.1 mg kg-1) metals in Malvella sherardiana, As (0.62±0.02 mg kg-1) metals in Mentha longifolia subsp. noeana were determined higher. While the toxic element (As and Pb) content of the studied species is low compared to WHO, Cd and Cr contents in some species are found to be high to WHO.

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Bu çalışmada Türkiye’nin Diyarbakır ve Mardin illerinde toplanmış, halk arasında yaygın olarak bitki çayı ve tibbi bitki olarak kullanılan Alcea rosea, Carduus pycnocephalus subsp. albidus, Euphorbia gaillardotii, E. macroclada, Hypericum triquetrifolium, Kickxia lanigera, Malvella sherardiana and Mentha longifolia subsp. noeana türlerinin ICP-OES tekniği ile (Al, As, B, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Se) eser element içeriği tayin edilmiştir. Türlerin tüm parçalarından oluşan örnekler ICP-OES ile analiz edilmeden önce porselen krozelerde kül edildilerek üzerine derişik nitrik asit ve hidrojen peroksit ilave edilerek hzırlanmıştır. Standart referans madde olarak NCS ZC73014 Çay Yaprağı kullanılarak metodun doğruluğu belirlenmiştir. Çalışılan tüm elementler için kalibrasyon eğrileri 0.05-1.5 mg L-1 konsantrasyon aralığında iyi doğrusallığa sahip olduğu belirlenmiştir. Korelasyon katsayısı (R) değerleri 0.9997' nin üzerinde olduğundan, doğrusal aralık kabul edilebilir olarak değerlendirilmiştir. Çalışılan her türün eser element içeriği bir biriyle karşılaştırılmıştır. Yapılan kıyaslamada; Carduus pycnocephalus subsp. albidus, türünde Cd (0.41±0.02 mg kg-1) ve Cu (13.9±0.9 mg kg-1), Euphorbia macroclada türünde B (22.4±1.6 mg kg-1), Hypericum triquetrifolium, Se (0.26±0.01 mg kg-1), Malvella sherardiana türünde Al (1424±109 mg kg-1), Cr (4.8±0.2 mg kg-1), Fe (980±67 mg kg-1) Mn (44.6±2.4 mg kg-1) Ni (7.5±0.3 mg kg-1) ve Pb (1.15±0.1 mg kg-1), Mentha longifolia subsp. noeana türünde As (0.62±0.02 mg kg-1) içeriği daha yüksek olduğu belirlenmiştir. Çalışılan türlerin toksik element (As ve Pb) içeriğinin WHO’ya göre düşük bulunurken, bazı türlerde Cd ve Cr içerikleri ise yüksek olduğu tespit edilmiştir.