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A hypothetical equation is derived from a method of column slicing previously described. When the derived equation is used to forecast the temperature-programmed gas chromatographic (TPGC) retention times of fatty acid methyl esters (FAMEs), the differences between the observed and calculated values are −2.6 to 3.4%. The greatest differences are found with higher programming rates and longer carbon chain lengths. When a flow-adjustment term is incorporated into the equation, the greatest difference between the observed and calculated retention times is reduced to approximately 2.7%. This equation can also accurately forecast the TPGC retention times of both saturated and unsaturated FAMEs from cauliflower seed oil.

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Kanisa Kittiratanapiboon, Narumon Jeyashoke, Kanit Krisnangkura, Forecasting Retention Times of Fatty Acid Methyl Esters in Temperature-Programmed Gas Chromatography, Journal of Chromatographic Science, Volume 36, Issue 11, November 1998, Pages 541–546, https://doi.org/10.1093/chromsci/36.11.541

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